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psbE-petL

LSC · 63933-65018 · 1086 bp

Back to Chenopodium

Pi 0.0121

MarkerSeek score 47.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1086
variable_sites: 31
pi: 0.0121
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACTAGTTAGAGTAGACA	50.0	34.6	57.8	57.2	1132-1140 bp	1.0	43.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	ACCGAAATAACTAGTTAGAGTAGACA	50.0	34.6	57.9	57.2	1209-1217 bp	1.0	43.0
psbE-petL_p3	ACGAATAACCAACCCGCAAT	ACCGAAATAACTAGTTAGAGTAGACA	45.0	34.6	58.2	57.2	1218-1226 bp	1.0	42.9
psbE-petL_p4	TGCTGACGAATAACCAACCC	ACCGAAATAACTAGTTAGAGTAGACA	50.0	34.6	58.2	57.2	1223-1231 bp	1.0	42.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	ACCGAAATAACTAGTTAGAGTAGACA	50.0	34.6	57.8	57.2	1217-1225 bp	1.0	42.9