Hotspot

trnT-UGU-trnL-UAA

LSC · 46409-47179 · 771 bp

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Pi 0.0178
MarkerSeek score 58.50
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
771
variable_sites
35
pi
0.0178
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG AGCCAATTAAGTCCGTAGCG 55.0 50.0 57.9 58.1 840-848 bp 1.0 83.0
trnT-UGU-trnL-UAA_p2 GACTCGAACCGATGACCATC AGCCAATTAAGTCCGTAGCG 55.0 50.0 58.2 58.1 871-879 bp 1.0 82.6
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 828-836 bp 1.0 81.5
trnT-UGU-trnL-UAA_p4 GACTCGAACCGATGACCATC CCGTAGCGTCTACCGATTTC 55.0 55.0 58.2 58.2 859-867 bp 1.0 81.1
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG GCCAATTAAGTCCGTAGCGT 55.0 50.0 57.9 58.4 839-847 bp 1.0 80.7