Hotspot

psbM-trnD-GUC

LSC · 28713-29498 · 786 bp

Back to Chenopodium

Pi 0.0117
MarkerSeek score 54.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
786
variable_sites
24
pi
0.0117
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCATTCTGGCTAACCGTTTT TCAATTGGTTAGAGCACCGC 40.0 50.0 55.3 58.6 949-956 bp 1.0 55.8
psbM-trnD-GUC_p2 TCATTCTGGCTAACCGTTTTT TCAATTGGTTAGAGCACCGC 38.1 50.0 56.1 58.6 949-956 bp 1.0 54.3
psbM-trnD-GUC_p3 AATCATTCTGGCTAACCGTT TCAATTGGTTAGAGCACCGC 40.0 50.0 55.0 58.6 951-958 bp 1.0 53.9
psbM-trnD-GUC_p4 ATCATTCTGGCTAACCGTTT TCAATTGGTTAGAGCACCGC 40.0 50.0 55.0 58.6 950-957 bp 1.0 53.9
psbM-trnD-GUC_p5 TCTGGCTAACCGTTTTTACA TCAATTGGTTAGAGCACCGC 40.0 50.0 54.9 58.6 945-952 bp 1.0 53.0