Hotspot

petN-psbM

LSC · 27930-28607 · 678 bp

Back to Chenopodium

Pi 0.0153

MarkerSeek score 50.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 37
pi: 0.0153
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	AAAACGGTTAGCCAGAATGA	50.0	40.0	57.6	55.3	715-727 bp	1.0	60.4
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAAACGGTTAGCCAGAATGA	50.0	38.1	57.6	56.1	716-728 bp	1.0	58.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	AAACGGTTAGCCAGAATGAT	50.0	40.0	57.6	55.0	714-726 bp	1.0	58.5
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	AACGGTTAGCCAGAATGATT	50.0	40.0	57.6	55.0	713-725 bp	1.0	58.5
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TGTAAAAACGGTTAGCCAGA	50.0	40.0	57.6	54.9	719-731 bp	1.0	57.6