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psbI-trnS-GCU

LSC · 7602-7800 · 199 bp

Back to Chenopodium

Pi 0.024

MarkerSeek score 52.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 12
pi: 0.024
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGACGCGAAGAA	GTCATTCGTACCGAGGGTTC	50.0	55.0	57.7	58.1	251-260 bp	1.0	84.2
psbI-trnS-GCU_p2	GTAATCCTGGACGCGAAGAA	AGTCATTCGTACCGAGGGTT	50.0	50.0	57.7	58.4	252-261 bp	1.0	81.2
psbI-trnS-GCU_p3	GTAATCCTGGACGCGAAGAA	TTTTCAACGGGGAGAGATGG	50.0	50.0	57.7	57.5	312-321 bp	1.0	79.5
psbI-trnS-GCU_p4	GTAATCCTGGACGCGAAGAA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.7	58.6	297-306 bp	1.0	78.7
psbI-trnS-GCU_p5	GTAATCCTGGACGCGAAGAA	GAGTCATTCGTACCGAGGGT	50.0	55.0	57.7	58.9	253-262 bp	1.0	77.6