Hotspot

rps16-trnQ-UUG

LSC · 5867-6475 · 609 bp

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Pi 0.0143

MarkerSeek score 62.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 609
variable_sites: 23
pi: 0.0143
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	683-703 bp	1.0	84.2
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	662-682 bp	1.0	84.2
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	673-693 bp	1.0	83.6
rps16-trnQ-UUG_p4	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	713-733 bp	1.0	83.2
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	692-712 bp	1.0	83.2