Hotspot

trnT-UGU-trnL-UAA

LSC · 46596-47384 · 789 bp

Back to Chenopodiastrum

Pi 0.0639

MarkerSeek score 80.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 50
pi: 0.0639
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	862 bp	1.0	83.7
trnT-UGU-trnL-UAA_p2	GACTCGAACCGATGACCATC	AGCCAATTAAGTCCGTAGCG	55.0	50.0	58.2	58.1	893 bp	1.0	83.3
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	850 bp	1.0	82.3
trnT-UGU-trnL-UAA_p4	GACTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	881 bp	1.0	81.9
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	55.0	50.0	57.9	58.4	861 bp	1.0	81.4