Hotspot

trnT-GGU-psbD

LSC · 30981-32192 · 1212 bp

Back to Chenopodiastrum

Pi 0.0506

MarkerSeek score 78.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1212
variable_sites: 50
pi: 0.0506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GCCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1189-1376 bp	1.0	86.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GCCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1192-1379 bp	1.0	86.3
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGCCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1191-1378 bp	1.0	86.3
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TAGCCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1194-1381 bp	1.0	86.2
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GCCCAGACCAACCTACAAAA	50.0	50.0	58.3	58.0	1166-1353 bp	1.0	84.8