Hotspot

trnE-UUC-trnT-GGU

LSC · 30419-30908 · 490 bp

Back to Chenopodiastrum

Pi 0.0637
MarkerSeek score 81.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
490
variable_sites
29
pi
0.0637
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 577-589 bp 1.0 86.8
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 575-587 bp 1.0 86.8
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 556-568 bp 1.0 86.5
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 554-566 bp 1.0 86.5
trnE-UUC-trnT-GGU_p5 GATGTCCTGAACCGCTAGAC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 574-586 bp 1.0 86.3