Hotspot

petN-psbM

LSC · 27893-28761 · 869 bp

Back to Chenopodiastrum

Pi 0.0558

MarkerSeek score 76.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 43
pi: 0.0558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGGAAGGAGAAGAAGAGGT	TCCAGATGTTTCTCGGGAGT	55.0	50.0	58.0	58.1	1952 bp	0.5	59.2
petN-psbM_p2	TTTTGTATCGTTTTGGCGGC	TCCAGATGTTTCTCGGGAGT	45.0	50.0	57.9	58.1	2114 bp	0.5	59.1
petN-psbM_p3	GGTACGTACTTGTGGGGTTC	TCCAGATGTTTCTCGGGAGT	55.0	50.0	57.9	58.1	1927 bp	0.5	58.8
petN-psbM_p4	CTTGGTACGTACTTGTGGGG	TCCAGATGTTTCTCGGGAGT	55.0	50.0	57.9	58.1	1930 bp	0.5	58.8
petN-psbM_p5	TGTTCCCCAGTTCAAATCCG	TCCAGATGTTTCTCGGGAGT	50.0	50.0	58.1	58.1	2059 bp	0.5	58.7