Hotspot

atpA-atpF

LSC · 11188-11256 · 69 bp

Back to Chenopodiastrum

Pi 0.0833

MarkerSeek score 77.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 5
pi: 0.0833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TTTCGTCTGCTCGAATGGTT	GTTTGGTGCGATGAACGAAA	45.0	45.0	57.8	57.6	116-125 bp	1.0	82.9
atpA-atpF_p2	TTTCGTCTGCTCGAATGGTT	CATGTTTGGTGCGATGAACG	45.0	50.0	57.8	58.4	119-128 bp	1.0	82.8
atpA-atpF_p3	TTTCGTCTGCTCGAATGGTT	TATTGGCATGTTTGGTGCGA	45.0	45.0	57.8	58.5	125-134 bp	1.0	82.1
atpA-atpF_p4	TTTCGTCTGCTCGAATGGTT	ATGTTTGGTGCGATGAACGA	45.0	45.0	57.8	58.5	118-127 bp	1.0	82.1
atpA-atpF_p5	TTTCGTCTGCTCGAATGGTT	ATATTGGCATGTTTGGTGCG	45.0	45.0	57.8	57.4	126-135 bp	1.0	81.1