Hotspot

ndhF-rpl32

SSC · 116113-117158 · 1046 bp

Back to Cheniella

Pi 0.00914

MarkerSeek score 69.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1046
variable_sites: 35
pi: 0.00914
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	TCAATGCTGCCCAATATCCC	45.0	50.0	58.0	58.0	1226-1255 bp	1.0	83.3
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	CAATGCTGCCCAATATCCCT	45.0	50.0	58.0	58.0	1225-1254 bp	1.0	83.3
ndhF-rpl32_p3	TTTGTTGCCGTCGGAAAAAG	TCAATGCTGCCCAATATCCC	45.0	50.0	57.8	58.0	1228-1257 bp	1.0	82.2
ndhF-rpl32_p4	TTTGTTGCCGTCGGAAAAAG	CAATGCTGCCCAATATCCCT	45.0	50.0	57.8	58.0	1227-1256 bp	1.0	82.1
ndhF-rpl32_p5	TGTTGCCGTCGGAAAAAGTA	AAAAGCTTTCAATGCTGCCC	45.0	45.0	58.0	57.8	1234-1263 bp	1.0	81.9