Hotspot

petD-rpoA

LSC · 80632-80872 · 241 bp

Back to Cheniella

Pi 0.0182

MarkerSeek score 55.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 8
pi: 0.0182
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	AGTGGACGTTTTAGAAAAGA	50.0	35.0	58.1	52.4	333-337 bp	1.0	43.6
petD-rpoA_p2	TGGTTGGGTATTGGAGCAAC	AGTGGACGTTTTAGAAAAGA	50.0	35.0	58.1	52.4	334-338 bp	1.0	43.6
petD-rpoA_p3	GTGGCTCTTTGGTTGGGTAT	AGTGGACGTTTTAGAAAAGA	50.0	35.0	57.8	52.4	343-347 bp	1.0	43.5
petD-rpoA_p4	CAGTAGCGACAACCGTCTTT	AGTGGACGTTTTAGAAAAGA	50.0	35.0	58.2	52.4	378-382 bp	1.0	43.2
petD-rpoA_p5	CCCATTTCGTCGTCCAGTAG	AGTGGACGTTTTAGAAAAGA	55.0	35.0	58.1	52.4	392-396 bp	1.0	43.1