Hotspot

psbZ-trnG-GCC

LSC · 38484-39102 · 619 bp

Back to Cheniella

Pi 0.00502

MarkerSeek score 59.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 619
variable_sites: 14
pi: 0.00502
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTGTTGTATTTGCTTCGCCG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	772-797 bp	1.0	81.4
psbZ-trnG-GCC_p2	TTGTATTTGCTTCGCCGGAT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	58.3	57.8	769-794 bp	1.0	80.3
psbZ-trnG-GCC_p3	CCTGTTGTATTTGCTTCGCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	773-798 bp	1.0	80.2
psbZ-trnG-GCC_p4	CTGTTGTATTTGCTTCGCCG	AAAAAGACGGAGGCGGATAG	50.0	50.0	58.1	57.7	807-832 bp	1.0	80.1
psbZ-trnG-GCC_p5	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	754-779 bp	1.0	79.5