Hotspot

trnT-GGU-psbD

LSC · 33807-35184 · 1378 bp

Back to Cheniella

Pi 0.00628
MarkerSeek score 63.90
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1378
variable_sites
27
pi
0.00628
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1520-1544 bp 1.0 80.2
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA GACCGGACCAACCTACAAAA 50.0 50.0 57.9 57.7 1534-1558 bp 1.0 80.1
trnT-GGU-psbD_p3 GCCCTTTTAACTCAGCGGTA TAGACCGGACCAACCTACAA 50.0 50.0 57.9 57.7 1536-1560 bp 1.0 79.7
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA GAGCAATAGACCGGACCAAC 50.0 55.0 57.9 58.3 1542-1566 bp 1.0 79.4
trnT-GGU-psbD_p5 GCCCTTTTAACTCAGCGGTA CAAAAACGAAACGGTCCCTC 50.0 50.0 57.9 57.6 1519-1543 bp 1.0 79.0