Hotspot

trnC-GCA-petN

LSC · 28871-30171 · 1301 bp

Back to Cheniella

Pi 0.0034
MarkerSeek score 60.50
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1301
variable_sites
22
pi
0.0034
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTTGTATCGTTTTGGCGGC AGCCCAAGCGAGACTTACTA 45.0 50.0 57.9 58.2 1394-1426 bp 1.0 81.8
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 1337-1369 bp 1.0 81.7
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 1356-1388 bp 1.0 79.5
trnC-GCA-petN_p4 TTTTGTATCGTTTTGGCGGC GCCCAAGCGAGACTTACTAT 45.0 50.0 57.9 57.1 1393-1425 bp 1.0 75.9
trnC-GCA-petN_p5 TTTTGTATCGTTTTGGCGGC ATTAAAGCAGCCCAAGCGAG 45.0 50.0 57.9 58.9 1402-1434 bp 1.0 75.8