Hotspot

atpF-atpH

LSC · 13799-14326 · 528 bp

Back to Cheniella

Pi 0.00374

MarkerSeek score 61.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 528
variable_sites: 9
pi: 0.00374
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGATGTCCAGTAAGCCAAGG	AGAGGGAAAAATCCGGGGTA	55.0	50.0	58.0	58.0	669-688 bp	1.0	84.0
atpF-atpH_p2	GGATGTCCAGTAAGCCAAGG	GAGGGAAAAATCCGGGGTAC	55.0	55.0	58.0	58.0	668-687 bp	1.0	83.8
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	AGAGGGAAAAATCCGGGGTA	50.0	50.0	57.9	58.0	690-709 bp	1.0	83.3
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GAGGGAAAAATCCGGGGTAC	50.0	55.0	57.9	58.0	689-708 bp	1.0	83.1
atpF-atpH_p5	GGATGTCCAGTAAGCCAAGG	AGGGAAAAATCCGGGGTACT	55.0	50.0	58.0	58.3	667-686 bp	1.0	81.7