Hotspot

trnQ-UUG-psbK

LSC · 7469-7914 · 446 bp

Back to Cheniella

Pi 0.00354

MarkerSeek score 47.90

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 9
pi: 0.00354
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	TGGATATGTTCTGGGACGGA	AGGCTTCAGGTAATTTCGCG	50.0	50.0	57.8	58.6	616-625 bp	1.0	75.4
trnQ-UUG-psbK_p2	TGGATATGTTCTGGGACGGA	GGCTTCAGGTAATTTCGCGA	50.0	50.0	57.8	58.6	615-624 bp	1.0	75.4
trnQ-UUG-psbK_p3	CCTTCGAATAACGGGACCAA	AGGCTTCAGGTAATTTCGCG	50.0	50.0	57.6	58.6	586-595 bp	1.0	73.7
trnQ-UUG-psbK_p4	AACCTTCGAATAACGGGACC	AGGCTTCAGGTAATTTCGCG	50.0	50.0	57.6	58.6	588-597 bp	1.0	73.7
trnQ-UUG-psbK_p5	CCTTCGAATAACGGGACCAA	GGCTTCAGGTAATTTCGCGA	50.0	50.0	57.6	58.6	585-594 bp	1.0	73.7