Hotspot

trnK-UUU-rps16

LSC · 4741-5725 · 985 bp

Back to Cheniella

Pi 0.00269

MarkerSeek score 63.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 12
pi: 0.00269
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.6	58.0	1110-1137 bp	1.0	79.0
trnK-UUU-rps16_p2	GAGTCGCACTTAAAAGCCGA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.6	58.0	1111-1138 bp	1.0	79.0
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	45.0	45.0	57.3	58.0	1108-1135 bp	1.0	77.7
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.6	58.2	1114-1141 bp	1.0	77.3
trnK-UUU-rps16_p5	GAGTCGCACTTAAAAGCCGA	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.6	58.2	1115-1142 bp	1.0	77.3