Hotspot

trnH-GUG-psbA

LSC · 341-715 · 375 bp

Back to Cheniella

Pi 0.0166

MarkerSeek score 52.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 24
pi: 0.0166
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	443-464 bp	1.0	83.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	457-478 bp	1.0	82.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	456-477 bp	1.0	82.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	458-479 bp	1.0	82.8
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CGGTCGAGGCTCCATCTATA	55.0	55.0	58.1	58.2	424-445 bp	1.0	82.4