Hotspot

ycf1

SSC · 125316-130946 · 5631 bp

Back to Chengiodendron

Pi 0.000533

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5631
variable_sites: 3
pi: 0.000533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCCTTCAAACGGCGATCT	AGGGATAACAGACTTCCGAGT	45.0	47.6	57.8	57.9	350 bp	1.0	70.0
ycf1_p2	CCTTCAAACGGCGATCTAGA	AGGGATAACAGACTTCCGAGT	50.0	47.6	57.4	57.9	347 bp	1.0	66.8
ycf1_p3	TCCTTCAAACGGCGATCTAG	AGGGATAACAGACTTCCGAGT	50.0	47.6	57.4	57.9	348 bp	1.0	66.8
ycf1_p4	TTTCCTTCAAACGGCGATCT	GGGATAACAGACTTCCGAGT	45.0	50.0	57.8	56.4	349 bp	1.0	65.7
ycf1_p5	TTTCCTTCAAACGGCGATCT	ACAGACTTCCGAGTTCTGAA	45.0	45.0	57.8	56.1	343 bp	1.0	63.9