Hotspot

ccsA

SSC · 115930-116901 · 972 bp

Back to Chengiodendron

Pi 0.00103

MarkerSeek score 59.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATGCCATCTTCTAAA	CACAGGGTTCTCATCAGTTCA	50.0	47.6	59.0	57.6	1167 bp	1.0	61.0
ccsA_p2	GCGGCATGCCATCTTCTAAA	CAGGGTTCTCATCAGTTCACA	50.0	47.6	59.0	57.6	1165 bp	1.0	61.0
ccsA_p3	GCGGCATGCCATCTTCTAAA	ACAGGGTTCTCATCAGTTCAC	50.0	47.6	59.0	57.6	1166 bp	1.0	61.0
ccsA_p4	GCGGCATGCCATCTTCTAAA	TCACAGGGTTCTCATCAGTTC	50.0	47.6	59.0	57.3	1168 bp	1.0	58.8
ccsA_p5	GCGGCATGCCATCTTCTAA	CAGGGTTCTCATCAGTTCACA	52.6	47.6	58.3	57.6	1165 bp	1.0	58.5