Hotspot

rpl32-trnL(UAG)

SSC · 114916-115754 · 839 bp

Back to Chengiodendron

Pi 0.00238

MarkerSeek score 60.10

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 839
variable_sites: 2
pi: 0.00238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	AGGGAAATCTCTTTTTACCGGG	TCCTAAGAGCAGCGTGTCTA	45.5	50.0	58.0	57.9	957-958 bp	1.0	62.9
rpl32-trnL(UAG)_p2	GGGAAATCTCTTTTTACCGGGA	TCCTAAGAGCAGCGTGTCTA	45.5	50.0	58.0	57.9	956-957 bp	1.0	62.8
rpl32-trnL(UAG)_p3	AGGGAAATCTCTTTTTACCGGG	AGCAGCGTGTCTACCAATTT	45.5	45.0	58.0	57.8	950-951 bp	1.0	62.4
rpl32-trnL(UAG)_p4	GGGAAATCTCTTTTTACCGGGA	AGCAGCGTGTCTACCAATTT	45.5	45.0	58.0	57.8	949-950 bp	1.0	62.4
rpl32-trnL(UAG)_p5	AGGGAAATCTCTTTTTACCGGG	CTAAGAGCAGCGTGTCTACC	45.5	55.0	58.0	57.8	955-956 bp	1.0	62.3