Hotspot

ndhF-rpl32

SSC · 114258-114735 · 478 bp

Back to Chengiodendron

Pi 0.00418

MarkerSeek score 58.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 478
variable_sites: 2
pi: 0.00418
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CGCTGTCCCATATCCTTTCC	50.0	55.0	58.0	58.1	660 bp	1.0	79.1
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CGCTGTCCCATATCCTTTCC	45.0	55.0	58.0	58.1	658 bp	1.0	79.0
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CGCTGTCCCATATCCTTTCC	45.0	55.0	58.0	58.1	659 bp	1.0	79.0
ndhF-rpl32_p4	TTTGTTGCCGTCGGAAAAAG	CGCTGTCCCATATCCTTTCC	45.0	55.0	57.8	58.1	663 bp	1.0	77.7
ndhF-rpl32_p5	GTTGCCGTCGGAAAAAGAAG	ACGCTGTCCCATATCCTTTC	50.0	50.0	58.0	57.4	661 bp	1.0	75.0