Hotspot

petA-psbJ

LSC · 65258-66209 · 952 bp

Back to Chengiodendron

Pi 0.0021

MarkerSeek score 60.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 2
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1080 bp	1.0	76.0
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1011 bp	1.0	75.8
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1010 bp	1.0	74.9
petA-psbJ_p4	TGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1087 bp	1.0	73.6
petA-psbJ_p5	TGAGAAGGTTCAATTGGCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.5	57.6	1012 bp	1.0	73.5