Hotspot

rpl32-trnL_UAG

SSC · 109505-110154 · 650 bp

Back to Cheirostylis

Pi 0.0557

MarkerSeek score 84.30

Primer available yes

Label rpl32-trnL_UAG

Feature Metadata

label_name: rpl32-trnL_UAG
feature_type: igs
region: SSC
length_bp: 650
variable_sites: 74
pi: 0.0557
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_UAG_p1	CTTTTTGACTGTCCGGTGGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	558-847 bp	1.0	90.7
rpl32-trnL_UAG_p2	CTTTTTGACTGTCCGGTGGA	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	899-1189 bp	1.0	90.5
rpl32-trnL_UAG_p3	CTTTTTGACTGTCCGGTGGA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	551-840 bp	1.0	90.2
rpl32-trnL_UAG_p4	CTTTTTGACTGTCCGGTGGA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	557-846 bp	1.0	90.1
rpl32-trnL_UAG_p5	CTTTTTGACTGTCCGGTGGA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	556-845 bp	1.0	90.1