Hotspot

clpP-psbB

LSC · 68686-69278 · 593 bp

Back to Cheirostylis

Pi 0.0649

MarkerSeek score 83.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 78
pi: 0.0649
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TCTACTCCGGGGGAAGATTC	ACACCCATATTCCAGGACCA	55.0	50.0	57.9	58.0	1816 bp	0.2	47.2
clpP-psbB_p2	TCTACTCCGGGGGAAGATTC	CAGATAGCTGCCAAGAAGCA	55.0	50.0	57.9	58.0	1649 bp	0.2	47.1
clpP-psbB_p3	TCTACTCCGGGGGAAGATTC	AGATAGCTGCCAAGAAGCAC	55.0	50.0	57.9	58.0	1648 bp	0.2	47.0
clpP-psbB_p4	TCTACTCCGGGGGAAGATTC	AACAGGGTCAGAGGGATCAA	55.0	50.0	57.9	58.0	1464 bp	0.2	47.0
clpP-psbB_p5	TCTACTCCGGGGGAAGATTC	ACAGGGTCAGAGGGATCAAA	55.0	50.0	57.9	58.0	1463 bp	0.2	47.0