Hotspot

rbcL-accD

LSC · 53967-54756 · 790 bp

Back to Cheirostylis

Pi 0.0604

MarkerSeek score 82.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 100
pi: 0.0604
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GGCTTCAGGGGGTATTCATG	CTTCCACCGGTATGTCCAAG	55.0	55.0	58.0	58.0	1007-1275 bp	1.0	91.7
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	CTTCCACCGGTATGTCCAAG	50.0	55.0	58.0	58.0	844-1112 bp	1.0	91.6
rbcL-accD_p3	GCACGACTACTTAACTGGGG	CTTCCACCGGTATGTCCAAG	55.0	55.0	58.0	58.0	1340-1608 bp	1.0	90.5
rbcL-accD_p4	GAGCGGTATTTGCCAGAGAA	CTTCCACCGGTATGTCCAAG	50.0	55.0	58.0	58.0	1380-1648 bp	1.0	90.2
rbcL-accD_p5	AAGAGCGGTATTTGCCAGAG	CTTCCACCGGTATGTCCAAG	50.0	55.0	58.0	58.0	1382-1650 bp	1.0	90.2