Hotspot

trnF_GAA-ndhJ

LSC · 45921-46497 · 577 bp

Back to Cheirostylis

Pi 0.0684

MarkerSeek score 76.90

Primer available yes

Label trnF_GAA-ndhJ

Feature Metadata

label_name: trnF_GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 72
pi: 0.0684
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF_GAA-ndhJ_p1	TACTGGTCGGGATAGCTCAG	TATTTACGCTCCCAGTGTGC	55.0	50.0	58.0	58.0	823-997 bp	1.0	91.6
trnF_GAA-ndhJ_p2	AGAGTACTGGTCGGGATAGC	TATTTACGCTCCCAGTGTGC	55.0	50.0	58.0	58.0	827-971 bp	0.8	78.4
trnF_GAA-ndhJ_p3	AGTACTGGTCGGGATAGCTC	TATTTACGCTCCCAGTGTGC	55.0	50.0	58.0	58.0	825-969 bp	0.8	78.4
trnF_GAA-ndhJ_p4	GAGTACTGGTCGGGATAGCT	TATTTACGCTCCCAGTGTGC	55.0	50.0	58.0	58.0	826-970 bp	0.8	78.4
trnF_GAA-ndhJ_p5	AGTACTGGTCGGGATAGCTC	CGAGGATTGGGACTCCATTG	55.0	55.0	58.0	58.0	874-1018 bp	0.8	78.1