Pi
0.0489
MarkerSeek score
83.10
Primer available
yes
Label
trnT_UGU-trnL_UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT_UGU-trnL_UAA_p1 | TCAATCATGGAAAGAGCCGG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 494-1068 bp | 1.0 | 90.7 |
| trnT_UGU-trnL_UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 437-1011 bp | 1.0 | 90.6 |
| trnT_UGU-trnL_UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 469-1043 bp | 1.0 | 89.4 |
| trnT_UGU-trnL_UAA_p4 | CTAACCTCTGAGCTAAGCGG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.5 | 58.0 | 429-1003 bp | 1.0 | 87.7 |
| trnT_UGU-trnL_UAA_p5 | CATGGAAAGAGCCGGCTATC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.5 | 58.0 | 489-1063 bp | 1.0 | 87.2 |