Hotspot

trnT_GGU-psbD

LSC · 30054-30920 · 867 bp

Back to Cheirostylis

Pi 0.0524

MarkerSeek score 84.40

Primer available yes

Label trnT_GGU-psbD

Feature Metadata

label_name: trnT_GGU-psbD
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 101
pi: 0.0524
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT_GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CTGCTACACCAAAACTGGGT	55.0	50.0	58.1	58.0	1305-1501 bp	1.0	89.4
trnT_GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GCCTAAGACTCCAGCAACTC	55.0	55.0	58.1	58.0	1394-1590 bp	1.0	89.2
trnT_GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAAGACTCCAGCAACTCCCA	55.0	50.0	58.1	58.0	1391-1587 bp	1.0	89.1
trnT_GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACAGCAATTGGGGC	55.0	45.0	58.1	58.0	1389 bp	0.2	47.2
trnT_GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAAAAACAGCAATTGGGGCA	55.0	45.0	58.1	58.0	1388 bp	0.2	47.2