Hotspot

trnC_GCA-petN

LSC · 26477-27342 · 866 bp

Back to Cheirostylis

Pi 0.061

MarkerSeek score 71.00

Primer available yes

Label trnC_GCA-petN

Feature Metadata

label_name: trnC_GCA-petN
feature_type: igs
region: LSC
length_bp: 866
variable_sites: 78
pi: 0.061
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.46
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC_GCA-petN_p1	AAGCACGATGATCCTAGGGA	CGTTTATTGCTACTGCGCTG	50.0	50.0	57.9	57.9	1589-2156 bp	1.0	90.7
trnC_GCA-petN_p2	AAGCACGATGATCCTAGGGA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	901-1452 bp	1.0	90.1
trnC_GCA-petN_p3	AAGCACGATGATCCTAGGGA	CTAAAGCCCACTTCTTCCCC	50.0	55.0	57.9	57.9	964-1515 bp	0.8	78.8
trnC_GCA-petN_p4	GGGTGTCGCCTAATCAACAA	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.2	57.9	1064-1624 bp	0.8	77.7
trnC_GCA-petN_p5	GGGTGTCGCCTAATCAACAA	CTAAAGCCCACTTCTTCCCC	50.0	55.0	58.2	57.9	439-983 bp	0.8	77.0