Hotspot

trnS_GCU-trnG_GCC

LSC · 7505-8013 · 509 bp

Back to Cheirostylis

Pi 0.0644

MarkerSeek score 84.20

Primer available yes

Label trnS_GCU-trnG_GCC

Feature Metadata

label_name: trnS_GCU-trnG_GCC
feature_type: igs
region: LSC
length_bp: 509
variable_sites: 73
pi: 0.0644
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS_GCU-trnG_GCC_p1	CAATCCGACGCTTTAGTCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	542-573 bp	1.0	59.9
trnS_GCU-trnG_GCC_p2	AATCCGACGCTTTAGTCCAC	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	541-572 bp	1.0	59.9
trnS_GCU-trnG_GCC_p3	CAATCCGACGCTTTAGTCCA	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	543-574 bp	1.0	58.8
trnS_GCU-trnG_GCC_p4	AATCCGACGCTTTAGTCCAC	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	542-573 bp	1.0	58.8
trnS_GCU-trnG_GCC_p5	CAATCCGACGCTTTAGTCCA	TTTACCACTAAACTATACCCGC	50.0	40.9	58.0	55.6	544-575 bp	1.0	57.1