Hotspot

psbK-psbI

LSC · 6797-7157 · 361 bp

Back to Cheirostylis

Pi 0.0717

MarkerSeek score 79.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 361
variable_sites: 55
pi: 0.0717
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGACGAAGAGTTTAAGAGTAAGCA	50.0	37.5	57.9	57.7	374-500 bp	1.0	56.9
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	AGACGAAGAGTTTAAGAGTAAGCA	45.0	37.5	58.3	57.7	371-497 bp	1.0	54.9
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	AGACGAAGAGTTTAAGAGTAAGCA	45.0	37.5	58.3	57.7	372-498 bp	1.0	54.9
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	AGACGAAGAGTTTAAGAGTAAGCA	45.0	37.5	58.3	57.7	373-499 bp	1.0	54.9
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TGTGTAGACGAAGAGTTTAAGAGT	50.0	37.5	57.9	57.4	379-505 bp	1.0	54.5