Hotspot

rps16-trnQ_UUG

LSC · 5819-6183 · 365 bp

Back to Cheirostylis

Pi 0.0722

MarkerSeek score 80.30

Primer available yes

Label rps16-trnQ_UUG

Feature Metadata

label_name: rps16-trnQ_UUG
feature_type: igs
region: LSC
length_bp: 365
variable_sites: 58
pi: 0.0722
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_UUG_p1	AAGTCGCACGTTGCTTTCTA	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.1	57.9	874-1201 bp	1.0	90.4
rps16-trnQ_UUG_p2	AAGTTTTGCTCGAGCTCCAT	CTTACAGCAGCTTGCCAAAC	45.0	50.0	57.8	57.9	1040-1367 bp	1.0	90.0
rps16-trnQ_UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	428-605 bp	1.0	89.2
rps16-trnQ_UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGCCCTAGTGACAACATG	45.0	50.0	58.1	57.9	569-580 bp	0.8	79.1
rps16-trnQ_UUG_p5	TCATGTCCTTCAAGTCGCAC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.2	57.9	885-1028 bp	0.8	79.0