Hotspot

rpoA

LSC · 77241-78248 · 1008 bp

Back to Cheirolophus

Pi 0.00198

MarkerSeek score 45.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 2
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CCTTCGGGGGATGGATCTAT	GGCTTCCGAGGTACAAGAAG	55.0	55.0	58.0	58.0	2047-2048 bp	1.0	79.8
rpoA_p2	TCTAGGGAACAGTCGCTTCA	GGCTTCCGAGGTACAAGAAG	50.0	55.0	58.1	58.0	1493-1494 bp	1.0	79.5
rpoA_p3	TTGGTTGGGTATTGGTGCAA	GGCTTCCGAGGTACAAGAAG	45.0	55.0	57.9	58.0	1593-1594 bp	1.0	79.5
rpoA_p4	TTTGGTTGGGTATTGGTGCA	GGCTTCCGAGGTACAAGAAG	45.0	55.0	57.9	58.0	1594-1595 bp	1.0	79.5
rpoA_p5	CCTTCGGGGGATGGATCTAT	TACTTGTGGCTTCCGAGGTA	55.0	50.0	58.0	58.1	2054-2055 bp	1.0	79.3