Hotspot

psbC

LSC · 32730-34151 · 1422 bp

Back to Cheirolophus

Pi 0.000703

MarkerSeek score 46.90

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TATTCGTGCTTGGATGGCG	GGGAACCGAAGTGACTCCTA	52.6	55.0	58.3	58.4	1500 bp	1.0	66.0
psbC_p2	GGTATTCGTGCTTGGATGGC	GGGAACCGAAGTGACTCCTA	55.0	55.0	59.3	58.4	1502 bp	1.0	65.8
psbC_p3	GATGGCGGCTCAAGATCAG	GGGAACCGAAGTGACTCCTA	57.9	55.0	58.4	58.4	1488 bp	1.0	65.4
psbC_p4	TATTCGTGCTTGGATGGCG	GGAACCGAAGTGACTCCTAC	52.6	55.0	58.3	57.3	1499 bp	1.0	64.4
psbC_p5	GGTATTCGTGCTTGGATGGC	GGAACCGAAGTGACTCCTAC	55.0	55.0	59.3	57.3	1501 bp	1.0	64.1