Hotspot

atpI

LSC · 23732-24475 · 744 bp

Back to Cheirolophus

Pi 0.00269

MarkerSeek score 60.30

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 2
pi: 0.00269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	GTCAAGTCGAGAAAGAGATGGT	GCCACGCACACATTACCTTA	45.5	50.0	57.8	58.6	869 bp	1.0	58.3
atpI_p2	GTCAAGTCGAGAAAGAGATGGT	TTAGCCACGCACACATTACC	45.5	50.0	57.8	58.6	872 bp	1.0	58.3
atpI_p3	AGTCAAGTCGAGAAAGAGATGG	GCCACGCACACATTACCTTA	45.5	50.0	57.6	58.6	870 bp	1.0	56.2
atpI_p4	AGTCAAGTCGAGAAAGAGATGG	TTAGCCACGCACACATTACC	45.5	50.0	57.6	58.6	873 bp	1.0	56.2
atpI_p5	AGTCGAGAAAGAGATGGTTGA	GCCACGCACACATTACCTTA	42.9	50.0	56.4	58.6	865 bp	1.0	55.0