Hotspot

atpF

LSC · 26216-27477 · 1262 bp

Back to Cheirolophus

Pi 0.00158

MarkerSeek score 59.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1262
variable_sites: 2
pi: 0.00158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGAGGGCAAAGTAATACAAAAAGA	ATTTCGTCGGCTTGAATGGT	33.3	45.0	56.5	58.2	1417 bp	1.0	40.1
atpF_p2	AGAGGGCAAAGTAATACAAAAAGA	GTCGGCTTGAATGGTTACCA	33.3	50.0	56.5	58.2	1412 bp	1.0	40.1
atpF_p3	AGTCTATCTATACGAGGAGATCC	ATTTCGTCGGCTTGAATGGT	43.5	45.0	55.5	58.2	1376 bp	1.0	40.1
atpF_p4	AGTCTATCTATACGAGGAGATCC	GTCGGCTTGAATGGTTACCA	43.5	50.0	55.5	58.2	1371 bp	1.0	40.1
atpF_p5	AGAGGGCAAAGTAATACAAAAAGA	TCGTCGGCTTGAATGGTTAC	33.3	50.0	56.5	58.3	1414 bp	1.0	40.1