Hotspot

rpl32-ndhF

SSC · 123978-125033 · 1056 bp

Back to Cheirolophus

Pi 0.00192

MarkerSeek score 61.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1056
variable_sites: 2
pi: 0.00192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1221-1236 bp	1.0	77.9
rpl32-ndhF_p2	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1219-1234 bp	1.0	77.8
rpl32-ndhF_p3	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1220-1235 bp	1.0	77.8
rpl32-ndhF_p4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1238-1253 bp	1.0	77.7
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.6	58.0	1236-1251 bp	1.0	77.6