Hotspot

petA-psbJ

LSC · 62484-63284 · 801 bp

Back to Cheirolophus

Pi 0.0025

MarkerSeek score 60.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 2
pi: 0.0025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	939-969 bp	1.0	76.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	929-959 bp	1.0	76.8
petA-psbJ_p3	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	945-975 bp	1.0	74.1
petA-psbJ_p4	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	944-974 bp	1.0	74.1
petA-psbJ_p5	GCCTTTTGTTCTTCTTCGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.2	57.6	928-958 bp	1.0	71.1