Hotspot

trnR-UCU-trnG-UCC

LSC · 29267-29527 · 261 bp

Back to Cheirolophus

Pi 0.0124

MarkerSeek score 63.70

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 261
variable_sites: 3
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	47.6	50.0	56.9	58.0	322-341 bp	1.0	68.1
trnR-UCU-trnG-UCC_p2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	47.6	55.0	56.9	58.1	325-344 bp	1.0	67.8
trnR-UCU-trnG-UCC_p3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	45.5	50.0	57.9	58.0	328-347 bp	1.0	67.4
trnR-UCU-trnG-UCC_p4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	45.5	55.0	57.9	58.1	331-350 bp	1.0	67.2
trnR-UCU-trnG-UCC_p5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	47.6	55.0	56.9	57.7	326-345 bp	1.0	65.6