Hotspot

psbM-trnD-GUC

LSC · 10499-11151 · 653 bp

Back to Cheirolophus

Pi 0.00155

MarkerSeek score 60.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 1
pi: 0.00155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TCTGATTCCCCTGGGATTGT	39.1	50.0	57.6	58.0	786-792 bp	1.0	53.1
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TCTGATTCCCCTGGGATTGT	40.0	50.0	54.5	58.0	780-786 bp	1.0	52.6
psbM-trnD-GUC_p3	ACTAAAATGAACAGTGCAGTAGC	TCTGATTCCCCTGGGATTGT	39.1	50.0	57.4	58.0	783-789 bp	1.0	51.7
psbM-trnD-GUC_p4	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	764-770 bp	1.0	51.1
psbM-trnD-GUC_p5	AGGAACTAAAATGAACAGTGCAG	TCTGATTCCCCTGGGATTGT	39.1	50.0	57.3	58.0	787-793 bp	1.0	51.0