Hotspot

ycf2

LSC · 88819-93222 · 4404 bp

Back to Cheirodendron

Pi 0.00136

MarkerSeek score 49.10

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 4404
variable_sites: 6
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGCCATGGTTCCTTACTTCG	TGGCGACTCATTATTTCGATGA	50.0	40.9	57.9	57.9	323 bp	1.0	63.9
ycf2_p2	GGTTCCTTACTTCGACAGGG	TGGCGACTCATTATTTCGATGA	55.0	40.9	57.6	57.9	317 bp	1.0	61.8
ycf2_p3	TGCCATGGTTCCTTACTTCG	TGAATCCTTTCTTCGGACAGA	50.0	42.9	57.9	56.7	304 bp	1.0	61.8
ycf2_p4	GCCATGGTTCCTTACTTCGA	TGGCGACTCATTATTTCGATGA	50.0	40.9	57.6	57.9	322 bp	1.0	61.6
ycf2_p5	TGCCATGGTTCCTTACTTCG	TGGCGACTCATTATTTCGATG	50.0	42.9	57.9	56.5	323 bp	1.0	60.4