Hotspot

ycf15

IRb · 95225-95527 · 303 bp

Back to Cheirodendron

Pi 0

MarkerSeek score 33.80

Primer available yes

Label ycf15

Feature Metadata

label_name: ycf15
feature_type: gene
region: IRb
length_bp: 303
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15_p1	TTAGCCGGAAAGACATGTGG	GGGATTGGCGATTTACCCAT	50.0	50.0	57.9	58.0	391 bp	1.0	79.1
ycf15_p2	GTGGCCATGAAAGAGGGATT	GGGATTGGCGATTTACCCAT	50.0	50.0	57.9	58.0	375 bp	1.0	78.8
ycf15_p3	TTAGCCGGAAAGACATGTGG	AGTCCGTTTTAGGGATTGGC	50.0	50.0	57.9	57.9	402 bp	1.0	78.1
ycf15_p4	TTAGCCGGAAAGACATGTGG	AACCTCTGGTAAAATGCCCG	50.0	50.0	57.9	58.2	453 bp	1.0	77.8
ycf15_p5	GTGGCCATGAAAGAGGGATT	AGTCCGTTTTAGGGATTGGC	50.0	50.0	57.9	57.9	386 bp	1.0	77.8