Hotspot

petD

LSC · 78959-80200 · 1242 bp

Back to Cheirodendron

Pi 0.00161

MarkerSeek score 58.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1242
variable_sites: 2
pi: 0.00161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TATTTCCCTTGAACGCTGCA	TCACTTTGGTTGAAGCGACT	45.0	45.0	57.8	57.7	1384-1385 bp	1.0	76.0
petD_p2	TTCCCTTGAACGCTGCAATA	TCACTTTGGTTGAAGCGACT	45.0	45.0	57.8	57.7	1381-1382 bp	1.0	76.0
petD_p3	TATTTCCCTTGAACGCTGCA	TGGTTGAAGCGACTATTCCC	45.0	50.0	57.8	57.6	1378-1379 bp	1.0	75.4
petD_p4	TTCCCTTGAACGCTGCAATA	TGGTTGAAGCGACTATTCCC	45.0	50.0	57.8	57.6	1375-1376 bp	1.0	75.4
petD_p5	TTCCCTTGAACGCTGCAATA	TCCCTAGATACACACGTCGT	45.0	50.0	57.8	57.6	1359-1360 bp	1.0	75.3