Hotspot

atpF

LSC · 12882-14166 · 1285 bp

Back to Cheirodendron

Pi 0

MarkerSeek score 26.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1285
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AGTCTATCTATAAGAGGAGATCA	50.0	34.8	57.7	52.5	1378-1379 bp	1.0	40.0
atpF_p2	TTGCTCAATACGTTCACGGA	AGTCTATCTATAAGAGGAGATCA	45.0	34.8	57.6	52.5	1417-1418 bp	1.0	40.0
atpF_p3	ACTAATTTCGTCGGCTCGAA	AGTCTATCTATAAGAGGAGATCA	45.0	34.8	57.4	52.5	1390-1391 bp	1.0	40.0
atpF_p4	TGCTCAATACGTTCACGGAT	AGTCTATCTATAAGAGGAGATCA	45.0	34.8	57.3	52.5	1416-1417 bp	1.0	40.0
atpF_p5	GGCTCGAATGGTTACCATGA	AGTCTATCTATAAGAGGAGATCAT	50.0	33.3	57.7	53.1	1378-1379 bp	1.0	40.0