Hotspot

trnL-CAA-ycf15

IRa · 147437-147756 · 320 bp

Back to Cheirodendron

Pi 0.0125

MarkerSeek score 60.10

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 320
variable_sites: 4
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	ACCCGACAGTACCCATTTTG	50.0	50.0	58.0	57.8	435 bp	1.0	79.1
trnL-CAA-ycf15_p2	TGAAATGGTAGACACGCGAG	ACCCGACAGTACCCATTTTG	50.0	50.0	58.0	57.8	437 bp	1.0	79.1
trnL-CAA-ycf15_p3	GCCTTGGTGGTGAAATGGTA	ACCCGACAGTACCCATTTTG	50.0	50.0	58.1	57.8	447 bp	1.0	78.5
trnL-CAA-ycf15_p4	AAATGGTAGACACGCGAGAC	TATTGAATTCACCCGACCCG	50.0	50.0	58.0	57.7	450 bp	1.0	78.1
trnL-CAA-ycf15_p5	TGAAATGGTAGACACGCGAG	TATTGAATTCACCCGACCCG	50.0	50.0	58.0	57.7	452 bp	1.0	78.1