Hotspot

psaA-ycf3

LSC · 44112-44797 · 686 bp

Back to Cheirodendron

Pi 0.00146

MarkerSeek score 59.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 1
pi: 0.00146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	864 bp	1.0	78.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TATGGCGCTTACTCCTGGTA	50.0	50.0	57.9	57.9	836 bp	1.0	78.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CTGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	879 bp	1.0	78.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGAGGCTTGGTTCGATCAAG	50.0	50.0	57.9	57.9	878 bp	1.0	78.4
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	866 bp	1.0	78.0