Hotspot

atpF-atpH

LSC · 14167-14548 · 382 bp

Back to Cheirodendron

Pi 0.0212

MarkerSeek score 66.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 8
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	57.9	58.2	464-467 bp	1.0	79.1
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	495-498 bp	1.0	78.5
atpF-atpH_p3	GTGGCCCAAAGAAACGAAAG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	465-468 bp	1.0	78.5
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	TGGCCTGGTTGTAGCATTAG	45.0	50.0	57.9	57.6	466-469 bp	1.0	77.7
atpF-atpH_p5	AGTGGCCCAAAGAAACGAAA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	58.2	58.2	466-469 bp	1.0	77.7